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Sigma-Aldrich Anti-Rantes Clone 1K17 Zoomab Rb
List Price $277.98 Your Price $277.98
Sigma-Aldrich Anti-Rantes Clone 1K17 Zoomab Rb; 25 μL - SIAL (Additional S&H or Hazmat Fees May Apply)
NETA PART: SIAL-ZRB2554-25UL
MFG.PART: ZRB2554-25UL
UNSPSC: 41116161
Manufacturer: Sigma-Aldrich
biological source
rabbit
Quality Level
200
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1K17, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 9.99 kDa
observed mol wt ~10/15 kDa
purified by
using Protein A
species reactivity
rat, human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
concentration
0.3 mg/mL
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
Internal
Protein ID accession no.
NP_002976.2
UniProt accession no.
P13501
greener alternative category
Aligned
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... CCL5(6352)
General description
C-C motif chemokine 5 (UniProt: P13501; also known as EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228 (TCP228), T-cell-specific protein RANTES) is encoded by the CCL5 (also known as D17S136E, SCYA5) gene (Gene ID: 6352) in human.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human RANTES.
Application
Quality Control Testing
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RANTES in human spleen tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human RANTES.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RANTES in lysate from rat spleen tissue.
Flow Cytometry Analysis: 1 µg from a representative lot detected RANTES in human peripheral blood mononuclear cells (PBMC) treated with 10 g/mL of Monensin for 14 hours.
Affinity Binding Assay: A representative lot of this antibody bound with RANTES peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected RANTES in human spleen and human bone marrow tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RANTES in human spleen tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human RANTES.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RANTES in lysate from rat spleen tissue.
Flow Cytometry Analysis: 1 µg from a representative lot detected RANTES in human peripheral blood mononuclear cells (PBMC) treated with 10 g/mL of Monensin for 14 hours.
Affinity Binding Assay: A representative lot of this antibody bound with RANTES peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected RANTES in human spleen and human bone marrow tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1K17 is a ZooMAb® rabbit recombinant monoclonal antibody that detects RANTES. It targets an epitope within 16 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| SKU | SIAL-ZRB2554-25UL |
|---|---|
| Supplier Part Number | ZRB2554-25UL |
| UM | PK |
| UNSPSC | 41116161 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Energy Star | No |
| Green | No |
| Controlled | N |