Sigma-Aldrich Anti-Ap-2# Clone 2J5 Zoomab Rabbit

Transcription factor AP-2 gamma (UniProt: Q92754; also known as AP2-gamma, Activating enhancer-binding protein 2 gamma, Transcription factor ERF-1) is encoded by the TFAP2C gene (Gene ID: 7022) in human. Transcription factor AP-2 gamma (AP-2 ) is a critical sequence-specific DNA-binding protein, belonging to the AP-2 family, involved in regulating gene expression during embryonic development and promoting cell proliferation and survival in cancerous tissues. AP-2 comprised of an N-terminal transactivation domain and a C-terminal DNA-binding and dimerization domain, enabling it to interact with enhancer elements and form homo- or heterodimers with other AP-2 family members. It binds to the consensus sequence 5′-GCCNNNGGC-3′, influencing the transcription of genes pivotal for development and differentiation. It is a retinoic acid-responsive gene involved in placental development and the progression of human breast cancer. AP-2 is required for development and maintenance of extra-embryonic membranes, trophectoderm. AP-2 is predominantly localized in the nucleus, where it interacts with various proteins, including proto-oncogene PELP1, enhancing signaling pathways such as RET, which are crucial in tumorigenesis. Its expression is notably high in tissues such as the placenta and mammary glands, and aberrant expression has been linked to breast cancer progression and therapy resistance. AP-2 is involved in pathways such as the regulation of the estrogen receptor and HER-2/neu proto-oncogene, implicating it in diseases like breast cancer and Char syndrome. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, J., et Al., (2021), Mol Oncol, 15(4), 1146-1161; Li, M., et Al., (2002), Gene, 301(1-2), 43-51; Nakaki, F., et Al., (2013), Nature, 501(7466), 222-226; Kołat, D., et Al., (2019), Biosci Rep, 39(3), BSR20181928; Williamson, J.A., et Al., (1996), Genomics, 35(2), 262-264).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 26 amino acids from the C-terminal region of human AP-2 .

Quality Control Testing

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected AP-2 in MCF7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AP-2 in T-47D cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound with AP-2 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected AP-2 in human skin tissue sections.

Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected AP-2 in MCF7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2J5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects AP-2 . It targets an epitope within 26 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

0.3 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.