Sigma-Aldrich Anti-#1-Antitrypsin Cl1K23 Zoomab Rb

Alpha-1-antitrypsin (UniProt: P01009; also known as Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1) is encoded by the SERPINA1 (also known as AAT, PI) gene (Gene ID: 5265) in human. The protein encoded by this gene belongs to the serpin family and is a strong serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Its pivotal role is to protect lower respiratory tract against proteolytic destruction by human leukocyte elastase. SERPINA protein is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to produce the mature protein. It is synthesized mainly in the liver and secreted to the bloodstream. It is predominantly found in the plasma, extracellular space, and extracellular matrix. Three Isoforms of -1-antitrypsin have been described that are produced by alternative splicing. Mutation in AAT gene will reduce the serum concentration of -1-antitrypsin and hence increase the risk of emphysema or liver disease. Recent studies suggest that AAT also exhibits anti-inflammatory and immunomodulatory functions, expanding its relevance beyond traditional protease inhibition. The complex formation of fatty acids or lipoproteins with native or modified AAT impacts its protease inhibitory action as well as its anti-inflammatory properties. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Segal, L. et Al., (2022). Cell Immunol. 375:104528; O′Brien, M.E., et Al. (2022). Int. J. Mol. Sci. 23(5);2441).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human 1-Antitrypsin.

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 1-Antitrypsin in HepG2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected 1-Antitrypsin in human plasma and in human liver tissue lysate.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected 1-Antitrypsin in human liver tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected 1-Antitrypsin in human liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1K23 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects 1-Antitrypsin. It targets an epitope within 23 amino acids from the Internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

0.3 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.