Sigma-Aldrich Anti-Fga Clone 4F6 Zoomab Rb Mono

Fibrinogen alpha chain (UniProt: P02671; also known as Fibrinopeptide A) is encoded by the FGA gene (Gene ID: 2243) in human. Fibrinogen alpha chain (FGA) is a critical component of fibrinogen, a plasma glycoprotein complex, synthesized in the liver, composed of three polypeptide chains: alpha (FGA), beta (FGB), and gamma (FGG). Fibrinogen plays a role in the stabilization of lesions, and it guides cell migration during re-epithelialization. The FGA chain upon cleavage by thrombin, forms monomers and together with FGB and FGG polymerize to form a matrix during the initial stages of wound repair. The conversion of fibrinogen to fibrin is essential for wound healing and tissue repair. FGA is involved in several pathways, including the Toll-like receptor pathway, influencing inflammation and immune response. Mutations in the FGA gene are linked to hereditary systemic amyloidosis and congenital afibrinogenemia, impacting fibrinogen′s assembly and secretion. Loss of FGA activity affects the acceleration of tumor growth and metastasis of lung cancer. It is a prognostic marker in liver hepatocellular carcinoma, lung squamous cell carcinoma, and pancreatic adenocarcinoma. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, H., et Al., (2020), Biol Reprod.,103(4), 779-790; Wang, M., et Al., (2020), Mol Cancer Res.,18(7), 943-954).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 25 amino acids from the internal region of human FGA.

Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FGA in HepG2 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FGA in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound with FGA peptide with at least one hundred thousand-fold (100,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FGA in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 4F6 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Fibrinogen alpha chain (FGA). It targets an epitope within 25 amino acids from the internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.