Sigma-Aldrich Anti-Npas4 Clone N408/79 25 Mig

Neuronal PAS domain-containing protein 4 (also known as Neuronal PAS4, HLH-PAS transcription factor NXF; UniProt: Q8CJH6) is encoded by the Npas4 (also known as Nxf) gene (Gene ID: 266734) in Rat. In human, the gene NPAS4 (neuronal PAS domain protein 4) is mapped to chromosome 11q13. It is also referred to as NXF (neuronal transcription factor) and LE-PAS (limbic-enriched PAS domain protein). The encoded protein belongs to the basic helix-loop-helix (bHLH)-PAS (Per-ARNT-Sim) protein family. It contains N-terminal bHLH domain (aa 1-53) and 2 PAS domains (aa 70-144 and aa 203-275). NPAS4 is mainly expressed in the brain and low levels are also present in the testis. The protein is localized in the nucleus and controls inhibitory and excitatory synapse formation. This action of NPAS4 depends on the neuronal cell type. NPAS4 also regulates the expression of brain-derived neurotrophic factor (BDNF), a neurotrophin required for neuronal viability, differentiation and synaptic plasticity. NPAS4 is associated with cognitive and social neurobehavior. In addition, it is involved in hippocampus- and amygdala-linked learning and memory. NPAS4 is also linked with psychiatric disorders, including bipolar disorder, autism spectrum disorder and cognition-associated disorders. This gene is also expressed during ischemic stroke and protects neurons against neurodegenerative insults.

Recombinant protein fragment corresponding to 597-802 amino acids from C-terminal half of rat NPAS4.

Quality Control Testing

Evaluated by Immunohistochemistry in rat cerebral cortex tissue sections

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected NPAS4 in rat cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone N408/79 is a mouse monoclonal antibody that specifically detects rat NPAS4. It targets an epitope within 260 amino acids from the C-terminal half.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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