Sigma-Aldrich Anti-Nkr-P1A/Cd161A Clone 10/78

Killer cell lectin-like receptor subfamily B member 1A (UniProt: ; also known as CD161a, NKR-P1A, Antigen 3.2.3, CD161 antigen-like family member A, Natural killer cell surface protein P1-3.2.3 (NKR-P1 3.2.3)) is encoded by the Klrb1a (also known as Nkrp1a) gene (Gene ID: 362443) in rat. Killer cell lectin-like receptor subfamily B (KLRB) members (NKRP1A/C/D) are a group of receptors primarily expressed on natural killer (NK) cells and some T cells. The expression of KLRB family members and can be influenced by cytokines, developmental cues, and the activation state of the immune cells. These are characterized by a C-type lectin-like domain that allows them to recognize specific carbohydrate structures on target cells. They typically consist of an extracellular region containing one or more C-type lectin-like domains, a single transmembrane domain, and a short cytoplasmic domain that may contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or activation motifs. Some KLRB members have immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that mediate inhibitory signaling. They function as receptors that can transmit either activating or inhibitory signals, influencing NK cell activity and T cell responses. KLRB members play a crucial role in distinguishing between healthy and abnormal (infected or transformed) cells, thereby regulating immune responses. Activation of KLRB members can lead to the cytotoxicity of target cells, while inhibitory signals help prevent autoimmunity and maintain self-tolerance. Studies suggest that NKRP1 molecules expressed on NK cells or transfectants were down-regulated by cross-linking with mAbs or cell surface ligands, and using this phenomenon as a functional assay for NKRP1-ligand interaction revealed that NKRP1F can recognize CLR-x. This antibody also detects other members of the KLRB family (UniProt: P27814 / Q99JB4, also known as NKR-P1C / NKR-P1D). KLRB members have been implicated in various diseases, including cancer, viral infections, and autoimmune disorders. Their role in immune surveillance makes them potential targets for immunotherapy, as enhancing KLRB-mediated cytotoxicity could improve anti-tumor responses. Conversely, dysregulation of KLRB expression or function may contribute to immune evasion by tumors or chronic infections. (Ref.: Aust, J.g., et Al. (2009). J Immunol. 183(1):106-16).

Rat NK cells.

Quality Control Testing

Evaluated by Flow Cytometry in CD rat splenocytes.

Flow Cytometry Analysis: 1.0 µg from a representative lot detected CD161a in one million CD rat splenocytes.

Tested Applications

Blocking of Interfering Antibodies: A representative lot blocked binding of the rat NKRP1-specific mAb 3.2.3 to NKR-P1A/CD161a in Blocking applications (Kraus, E., et Al. (1996). Eur J Immunol. 26(11):2582-6).

Immunoprecipitation Analysis: A representative lot immunoprecipitated NKR-P1A/CD161a in radio-immunoprecipitation application (Kraus, E., et Al. (1996). Eur J Immunol. 26(11):2582-6).

Immunohistochemistry Applications: A representative lot detected NKR-P1A/CD161a in Immunohistochemistry applications (Schwartzkopff, J., et Al. (2010). Mol Vis. 2:16:1928-35).

Flow Cytometry Analysis: A representative lot detected NKR-P1A/CD161a in Flow Cytometry applications (Kraus, E., et Al. (1996). Eur J Immunol. 26(11):2582-6; Sedgwick, J.D., et Al. (1998). J Immunol. 160(11):5320-30; Schwartzkopff, J., et Al. (2010). Mol Vis. 2:16:1928-35).

Immunofluorescence Analysis: A representative lot detected NKR-P1A/CD161a in Immunofluorescence applications (Kraus, E., et Al. (1996). Eur J Immunol. 26(11):2582-6).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 10/78 is a mouse monoclonal antibody that detects NKR-P1A/CD161a. It targets an epitope from the extracellular domain.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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