Sigma-Aldrich Anti-Cd79A/Mb-1 Clone Hm47 25 Mig

B-cell antigen receptor complex-associated protein alpha chain (UniProt: P11912; also known as Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein) is encoded by the CD79A (also known as IGA, MB1) gene (Gene ID: 973) in human. CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling. This mouse monoclonal antibody clone, HM47, reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Thyroglobulin conjugated linear peptide corresponding to 15 amino acids from the C-terminal extracellular domain of human .

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:500 Dilution from a representative lot detected CD79A/MB-1 in Raji cell lysate.

Tested Applications

Flow Cytometry Analysis: 1.0 µg from a representative lot detected CD79A in one million Daudi cells.

Western Blotting Analysis: A representative lot detected CD79A/MB-1 in Western Blotting application (van Noesel, C.J., et Al. (1991). J Immunol. 146(11):3881-8).

Immunoprecipitation Analysis: A representative lot detected CD79A/MB-1 in Immunoprecipitation application (van Noesel, C.J., et Al. (1991). J Immunol. 146(11):3881-8; Mason, D.Y., et Al. (1991). J Immunol. 147(11):2474-82).

Immunohistochemistry Applications: A representative lot detected CD79A/MB-1 in Immunohistochemistry applications (Mason, D.Y., et Al. (1991). J Immunol. 147(11):2474-82).

Flow Cytometry Analysis: A representative lot detected CD79A/MB-1 in Flow Cytometry applications (Mason, D.Y., et Al. (1991). J Immunol. 147(11):2474-82).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone HM47 is a mouse recombinant monoclonal antibody that detects CD79A/MB-1. It targets an epitope within 15 amino acids from the C-terminal extracellular domain.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.