Sigma-Aldrich Anti-Vimentin Clone Ln-6 1 mL

Clone LN-6 is a mouse monoclonal antibody that specifically detects Vimentin.

Vimentin (UniProt: P08670) is encoded by the VIM gene (Gene ID: 7431) in human. Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. It is the major subunit protein of the intermediate filaments of mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. It is usually expressed by cells undergoing cell-to-cell adhesion, EMT, and cytoskeleton remodeling. It is also involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is also involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It has also been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin consists of a central -helical rod domain. The N-terminal part of the rod domain is a coil 1 motif that is organized into sequences of seven amino acid residues, where every first and fourth residue is hydrophobic. The C-terminal half of the rod domain has a coil 2 motif that shows a hydrophobic pattern. The vimentin structure shown to be dynamic in cells and can be rearranged to form filaments or the soluble tetrameric protein. The head and tail domains of vimentin contain over 35 phosphorylation sites and phosphorylation of its serine residues is reported to inhibit its subunit polymerization, thus promoting the disassembly of vimentin filaments and increasing its solubility. These phosphorylations also regulate protein-protein interactions and intracellular signaling. (Ref.: Stathopoulos, E., et Al. (1989). J Histochem Cytochem. 37(9):1363-70; Zhang, X., et Al. (2014). PLoS One. 9(4):e94658; Shukla, P., et Al. (2015). BMC Genomics. 16:16:944; Pal, B., et Al. (2017). Nat Commun. 8(1):1627; Calle, A., et Al. (2019). Stem Cell Res Ther. 10(1):23).

Quality Control Testing

Evaluated by Immunohistochemistry in human tonsil tissue sections

Immunohistochemistry (Paraffin) Analysis: A 1:5 dilution from a representative lot detected Vimentin in human tonsil tissue sections.




Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:5 dilution from a representative lot detected Vimentin in human testis tissue sections.

Immunocytochemistry Analysis: A representative lot detected Vimentin in Immunocytochemistry application (Zhang, X., et Al. (2014). PLoS One. 9(4):e94658; Hayashi, Y., et Al. (2023). Cancer Cell Int. 23(1):57; Rocha Teixeira Netto, A., et Al. (2023). Methods Protoc. 6(3):50).

Immunofluorescence Analysis: A representative lot detected Vimentin in Immunofluorescence application (Pal, B., et Al. (2017). Nat Commun. 8(1):1627; Hayashi, Y., et Al. (2023). Cancer Cell Int. 23(1):57).

Immunoprecipitation Analysis: A representative lot detected Vimentin in Immunoprecipitation application (Stathopoulos, E., et Al. (1989). J Histochem Cytochem. 37(9):1363-70).

Flow Cytometry Analysis: A representative lot detected Vimentin in Flow Cytometry application (Calle, A., et Al. (2019). Stem Cell Res Ther. 10(1):23).

Immunohistochemistry Application: A representative lot detected Vimentin in Immunohistochemistry application (Stathopoulos, E., et Al. (1989). J Histochem Cytochem. 37(9):1363-70; Pal, B., et Al. (2017). Nat Commun. 8(1):1627).

Western Blotting Analysis: A representative lot detected Vimentin in Western Blotting application (S Shukla, P., et Al. (2015). BMC Genomics. 16:16:944; Provenzano, F., et Al. (2022). Cells. 11(23):3923).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Mouse monoclonal antibody IgM as culture supernatant without azide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nuclei from adult human thymic biopsy specimens.

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.