Sigma-Aldrich Anti-Rnf43 Clone Ggtrnf 8D6 25 Mig

E3 ubiquitin-protein ligase RNF43 (UniProt: Q68DV7; also known as RING finger protein 43, RING-type E3 ubiquitin transferase RNF43) is encoded by the RNF43 gene (Gene ID: 54894) in human. RNF43 plays a critical role in negatively regulating Wnt signaling, a pathway essential for cell proliferation, differentiation, stem cell maintenance, embryonic development and tumorigenesis. RNF43 contains an N-terminal extracellular domain (aa 24-197), a transmembrane domain (aa 198-218), and a large cytoplasmic region (aa 219-783) that contains an atypical RING-type zinc finger (aa 272-313) and a pro-rich (aa 569-760) region. The RING finger domain allows it to facilitate the transfer of ubiquitin from an E2 enzyme to target proteins. This activity is central to its role in modulating the Wnt signaling pathway, as RNF43 ubiquitinates Frizzled receptors, leading to their degradation and thus to downregulation of the Wnt signaling. Phosphorylation of conserved serines also plays a role in regulating the function and oncogenic potential of RNF43. Missense mutations, or truncations of RNF43 have been implicated in several cancers, including colorectal, gastric, and pancreatic cancers. In addition, RNF43 mutations are associated with cystic fibrosis-related intestinal cancer and serrated polyposis syndrome, highlighting its importance in maintaining epithelial integrity. (Ref: Tsukiyama, T., et. Al. (2020). Nat. Commun.15, 11(1): 4586)

Ovalbumin-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human RNF43.

Quality Control Testing

Evaluated by Western Blotting in lysate from HCT116 cells expressing RNF43.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RNF43 in lysate from HCT116 cells transfected with human RNF43.

Tested Applications

Immunohistochemistry Applications: A representative lot detected RNF43 in Immunohistochemistry application (Neumeyer, V., et Al. (2019). Carcinogenesis. 40(4); 551-559).

Western Blotting Analysis: A representative lot detected RNF43 in Western Blotting application (Li, S. et Al. (2023). PLoS One. 18(4): 1-16).

Immunocytochemistry Analysis: A representative lot detected RNF43 in Immunocytochemistry application (Niu J. et Al. (2021). Neuron.109(19); 3104-3118).

Immunoprecipitation Analysis: A representative lot Immunoprecipitated RNF43 (Tsukiyama, T., et. Al. (2020). Nat. Commun. 15, 11(1): 4586; Niu J. et Al. (2021). Neuron. 109(19); 3104-3118).

Immunofluorescence Analysis: A representative lot detected RNF43 in Immunofluorescence application (Li, S. et Al. Oncogene (2020). 39(17):3458-3472).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone GGTRNF 8D6 is a rat recombinant monoclonal antibody that detects RNF43. It targets an epitope within 20 amino acids from the C-terminal half.

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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