Sigma-Aldrich Lum1 High-Metastatic Murine Cell Line

Tumor metastasis is one of the most life-threatening events in cancer and often leads to inevitable cancer growth that cannot be effectively treated. Treatment options after metastasis often involve slowing tumor growth or relieving harsh symptoms. The process of metastasis, known as the metastatic cascade, involves a series of events in which cancer cells break away from the primary tumor and begin growing in other tissues. This process proceeds by cancer cells moving through blood vessels or lymph nodes and spreading via the circulatory system to eventually colonize other more distant tissues. The importance of understanding metastasis remains continually relevant and requires a variety of models for understanding the underlying mechanisms. LuM1 (SCC654) and NM11 (SCC655) were developed together to provide two similar cell models with differing metastatic potentials. NM11 was selected as low-metastatic cell line and is derived from murine colon adenocarcinoma while LuM1 was developed through repeated tumor transplantation until metastatic potential of the cells had changed visibly through lung tumor nodule formation.

The LuM1 and NM11 cell lines have been utilized for metastasis, migration, and invasion assays. LuM1 and NM11 have been useful in understanding Matrix MetalloProteinase (MMP) protein expression and their relation to metastatic potential and tumor progression.

Source: Derived from mouse colon adenocarcinoma that was transplanted into a BALB/c mouse. Resulting tumor was repeatedly transplanted into new mice until metastatic potential of the tumors were visible through lung nodule formation.

References

Sakata K, Kozaki K, Iida K, Tanaka R, Yamagata S, Utsumi KR, Saga S, Shimizu S, Matsuyama M. 1996. Establishment and characterization of high- and low-lung-metastatic cell lines derived from murine colon adenocarcinoma 26 tumor line. Japanese journal of cancer research : Gann. 87(1):78–85.

Taha EA, Chiharu Sogawa, Yuka Okusha, Kawai H, May Wathone Oo, Abdellatif Elseoudi, Lu Y, Hitoshi Nagatsuka, Kubota S, Ayano Satoh, et Al. 2020. Knockout of MMP3 Weakens Solid Tumor Organoids and Cancer Extracellular Vesicles. Cancers. 12(5):1260–1260.

LuM1 cells have been utilized for metastasis, migration, and invasion assays. LuM1 cells have been useful in understanding Matrix MetalloProteinase (MMP) protein expression and their relation to metastatic potential and tumor progression.

The cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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