Sigma-Aldrich PET-46 EK/LIC VECTOR KIT 20 T

The PET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in PET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell. T7 RNA polymerase is SO selective and active that, when fully induced, almost all of the cell′s resources are converted to target gene expression; the desired product can comprise more than 50% of the total cell protein a few hours after induction. All of the PET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. The PET-46 Ek/LIC Vector is a LIC-compatible derivative of PET-45b(+). The plasmid contains a strong T7lac promoter and an amino-terminal HIS TAG^® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pCDF-2 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.

Toxicity: Multiple Toxicity Values, refer to MSDS

Kim, J.S. and Raines, R.T. 1993. Protein Science 2, 348.

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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