Sigma-Aldrich Anti-Globo H Ceramide/Ghcer Clvk9

Globo H-KLH conjugate

Quality Control Testing

Evaluated by Immunohistochemistry in human liver cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Globo H-ceramide (GHCer) in human liver cancer tissue sections.

Tested Applications

Inhibition Assay: A representative lot of this antibody inhibited blood vessel formation in Matrigel plug and reduced hemoglobulin accumulation. (Wang, S-H., et Al. (2022). J Biomed Sci. 29:105).

Electron Microscopy: A representative lot detected Globo H ceramide/GHCer in Electron Microscopy application (Wang, S-H., et Al. (2022). J Biomed Sci. 29:105).

DOT Blot: A representative lot detected Globo H ceramide/GHCer in DOT Blot application (Wang, S-H., et Al. (2022). J Biomed Sci. 29:105).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Globo H ceramide/GHCer in Immunoprecipitation application (Kudryashov, V., et Al. (1998). Glycoconj J. 15(3):243-9; Wang, S-H., et Al. (2022). J Biomed Sci. 29:105).

Immunofluorescence Analysis: A representative lot detected Globo H ceramide/GHCer in Immunofluorescence application (Lo, T-C., et Al. (2023). Dev Cell. 58(22):2447-2459).

Flow Cytometry Analysis: A representative lot detected Globo H ceramide/GHCer in Flow Cytometry application (Lo, T-C., et Al. (2023). Dev Cell. 58(22):2447-2459).

Immunocytochemistry Analysis: A representative lot detected Globo H ceramide/GHCer in Immunocytochemistry application (Kudryashov, V., et Al. (1998). Glycoconj J. 15(3):243-9).

ELISA: A representative lot detected Globo H ceramide/GHCer in an ELISA application (Kudryashov, V., et Al. (1998). Glycoconj J. 15(3):243-9).

Immunohistochemistry Analysis: A representative lot detected Globo H ceramide/GHCer in Immunohistochemistry application (Wang, S-H., et Al. (2022). J Biomed Sci. 29:105).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone VK9 is a mouse monoclonal antibody that specifically detects Globo H-ceramide (GHCer).

Purified mouse monoclonal antibody IgG3 in PBS without preservatives.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Glycosphingolipids (GSLs) are a heterogeneous class of membrane glycolipids consisting of a ceramide backbone covalently linked to a glycan moiety and are involved in many cellular processes. Globo H (Fuc 1 2Galβ1 3GalNAcβ13Gal 14Galβ1 4Glc) is a carbohydrate structure, which is synthesized by specific glycosyltransferases, and shows enhanced expression in many human carcinomas. Globo H-ceramide (GHCer) is a glycosphingolipid and consists of a ceramide backbone with a carbohydrate chain that includes the Globo H, and serves as a critical tumor-associated carbohydrate antigen. It is predominantly expressed in various epithelial cancers, including breast, pancreatic, and colorectal cancers. GHCer is involved in cell signaling pathways that regulate tumor progression, immune evasion, and angiogenesis. Its expression is notably upregulated in malignant cells compared to normal tissues, making it a potential biomarker for cancer diagnosis and prognosis. Subcellularly, Globo H-ceramide localizes in lipid rafts and membrane microdomains, facilitating interactions with signaling molecules and receptors. Its involvement in disease is highlighted by its role in promoting cancer cell proliferation and metastasis, as well as its potential as a target for immunotherapeutic strategies aimed at enhancing anti-tumor immunity. This mAb VK-9 is suitable for multiple applications including western blotting, immunocytochemistry, immunoprecipitation, flow cytometry ELISA, and inhibition studies. It does not cross react with many related structures, such as galactosylgloboside, globoside, H type 1, H type 2 blood group structures or fucosyl-gangliotetraosyl ceramide, but reacts weakly with globo A ceramide. It recognizes globo H-ceramide and human tumor cells expressing globo H. (Ref.: Kudryashov, V., et Al. (1998). Glycoconj J. 15(3):243-9; Wang, S-H., et Al. (2022). J Biomed Sci. 29:105; Lo, T-C., et Al. (2023). Dev Cell. 58(22):2447-2459.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.