Sigma-Aldrich Anti-Lamp2 Clone Gl2A7 25 Mig

Lysosome-associated membrane glycoprotein 2 (UniProt: P17047; also known as LAMP-2; Lysosome-associated membrane protein 2) is encoded by the Lamp2 (also known as Lamp-2) gene (Gene ID: 16784) in murine species. LAMP-2 is a single-pass type I, heavily glycosylated, membrane protein found in plasma membrane, endosomes, and lysosomes. It is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains a lumenal domain (aa 26-379), a transmembrane domain (aa 380-404), and a short cytoplasmic tail (aa 405-415). It is an important component of lysosomal membranes and is involved in both autophagy and lysosomal membrane permeabilization. LAMP-2 plays an important role in chaperone-mediated autophagy. It is required for the fusion of autophagosomes with lysosomes during autophagy. It also plays a role in lysosomal protein degradation in response to starvation. It functions by binding target proteins, such as GAPDH and MLLT11, and targets them for lysosomal degradation. In cancer cells, chronic acidosis-induced upregulation of LAMP-2 has been reported that protects these cells from acid-induced hydrolysis and promotes their survival via chaperone-mediated autophagy. Loss of LAMP-2 is reported to aggravate oxidative stress through the obstruction of reactive oxygen species clearance. Three different isoforms of LAMP-2 have been described that are produced by alternative splicing. Mutations in LAMP2 gene have been linked to Danon disease that is characterized by cardiomyopathy, vascular myopathy, and mental retardation. It is often associated with accumulation of glycogen in muscle and lysosomes. (Ref.:Granger, B.L., et Al. (1990). J Biol Chem. 265(20):12036-43; Gondre-Lewis, T.A., et Al. (2001). J Immunol. 166(11):6657-64; Yan, J., et Al. (2005). Int Immunol. 17(7):869-77; Nag, S., et Al. (2018). J Cell Sci. 131(18):jcs216226).

Mouse liver lysosomes.

Quality Control Testing

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:125 dilution from a representative lot detected LAMP2 in NIH3T3 cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected LAMP2 in Western Blotting application (Granger, B.L., et Al. (1990). J Biol Chem. 265(20):12036-43; Nag, S., et Al. (2018). J Cell Sci. 131(18):jcs216226).

Immunocytochemistry Analysis: A representative lot detected LAMP2 in Immunocytochemistry application (Gondre-Lewis, T.A., et Al. (2001). J Immunol. 166(11):6657-64).

Immunoprecipitation Analysis: A representative lot detected LAMP2 in Immunoprecipitation application (Granger, B.L., et Al. (1990). J Biol Chem. 265(20):12036-43).

Immunofluorescence Analysis: A representative lot detected LAMP2 in Immunofluorescence application (Granger, B.L., et Al. (1990). J Biol Chem. 265(20):12036-43; Gondre-Lewis, T.A., et Al. (2001). J Immunol. 166(11):6657-64; Yan, J., et Al. (2005). Int Immunol. 17(7):869-77; Nag, S., et Al. (2018). J Cell Sci. 131(18):jcs216226; Giocoechea, L., et Al. (2021). STAR Protoc. 3(1):101068).

Immunohistochemistry Analysis: A representative lot detected LAMP2 in Immunohistochemistry application (Giocoechea, L., et Al. (2021). STAR Protoc. 3(1):101068).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone GL2A7 is a rat monoclonal antibody that specifically detects mouse LAMP2 (CD107b).

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.