Sigma-Aldrich Milli ╡-Angiogenesis Inhibition Assay

Angiogenesis, the formation of new blood vessels from a pre-existing vascular network, occurs normally in development and is critical for a majority of the vessel formation that occurs during embryogenesis, tissue generation, and wound healing. However, abnormal blood vessel growth, either excessive or insufficient, can be the underlying cause of many deadly and debilitating diseases including cancer, cardiovascular disease, stroke, and diabetic and age-related blindness. Identification of specific compounds that promote or inhibit angiogenesis may provide promising treatments for these diseases.

Tubular formation is a multi-step process involving cell adhesion, migration, differentiation, and growth. Millipore′s TM=["Millicell"] µ-Angiogenesis Inhibition Assay provides an efficient system for the rapid and accurate identification of factors that inhibit tube formation of endothelial cells. The µ-Angiogenesis slide, with enhanced optical capabilities in a microscale format, allows for real time and continuous monitoring of tube formation in a simplifed and reproducible manner.

1. Millicell^® µ-Angiogenesis Slides (Part No. CS203030) should be stored at room temperature until ready to use.2. ECMatrix Gel Solution (Part No. 90060) should be stored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one week. Do not refreeze after thawing as this may affect efficacy.3. Calcein AM (Part No. CS202541) is provided as 50 µg lyophilized powder. To prepare 1 mm Calcein AM Stock Solution, briefly centrifuge before opening tube and then add 50 µL sterile DMSO (not provided). Vortex vigorously. Centrifuge briefly to collect solution. Unused Calcein AM solution may be stored in aliquots at -20°C for up to 1 month.4. D, L-Sulforaphane (Part No. CS203032) is provided as 1 mg lyophilized powder. To prepare a 50 mm Stock Solution, dissolve powder in 112 µL sterile DMSO. Store reconstituted D, L-Sulforaphane solution at -20°C for up to 3 months. Avoid repeated freeze/thaw cycles.

1. Millicell^® µ-Angiogenesis Slides: (Part No. CS203030) Five tissue culture treated slides. Each slide contains 15 wells. 2. ECMatrix Gel Solution: (Part No. 90060) One 1 mL vial. 3. Calcein AM: (Part No. CS202541) One 50 µg vial. 4. D, L-Sulforaphane: (Part No. CS203032) One 1 mg vial.

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