Sigma-Aldrich Protein Delivery System Human Follista&

Name: Sigma-Aldrich Protein Delivery System Human Follista&
SKU: SIAL-938009-1EA
Price: 247.50 USD

List Price $247.50 Your Price $247.50

Sigma-Aldrich Protein Delivery System Human Follista& - SIAL (Additional S&H or Hazmat Fees May Apply)

NETA PART: SIAL-938009-1EA

MFG.PART: 938009-1EA

UNSPSC: 12352202

Manufacturer: Sigma-Aldrich

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Description

biological source

(Spodoptera frugiperda (Sf9) cell culture)

Quality Level

100

form

(monodisperse crystals)

packaging

1 ea of 25 μg

technique(s)

: suitable using 3D bioprinting | cell culture

impurities

<0.06 EU/mL Endotoxin

color

colorless

storage temp.

2-8°C

General description

Protein delivery system, Human Follistatin, PODS contains the polyhedrin protein co-crystalized with Human Follistatin. Follistatin is an autocrine, activin-binding protein that is ubiquitously expressed with highest expression levels being in the ovary and skin. Follistatin negatively regulates the signaling of Transforming Growth Factor β (TGF-β) family members, such as activin, bone morphogenic proteins (BMP), myostatin, Growth Differentiation Factor 11 (GDF-11), and TGF-β1. Follistatin functions as an antagonist by binding TGF-β family members to block interaction with their signaling receptors. Follistatin also inhibits the secretion of Follicle-Stimulating Hormone (FSH) from the anterior pituitary.

Application

PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.

PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.

Features and Benefits

Protein delivery system, PODS human follistatin comes with molecular weight of 36.7 kDa, length 333 aa and endotoxin level <0.06 EU/mL. PODS co-crystals may be reconstituted at 200 million co-crystals/mL in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
PODS provides durability, stability AND sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.

Legal Information

PODS is a registered trademark of Cell Guidance Systems Ltd

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c