We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Sigma-Aldrich Maleimide-Peg2-Biotin
List Price $286.25 Your Price $286.25
Sigma-Aldrich Maleimide-Peg2-Biotin - SIAL (Additional S&H or Hazmat Fees May Apply)
NETA PART: SIAL-SML4178-50MG
MFG.PART: SML4178-50MG
UNSPSC: 12352200
Manufacturer: Sigma-Aldrich
Quality Level
100
assay
≥98% (HPLC)
form
powder
color
white to beige
solubility
DMSO: 2 mg/mL, clear
storage temp.
-10 to -25°C
SMILES string
O=C(N1CCC(NCCOCCOCCNC(CCCC[C@H]2[C@@]3([C@]([H])(CS2)NC(N3)=O)[H])=O)=O)C=CC1=O
General description
Maleimide-PEG2-biotin, a maleimide-conjugated biotin molecule with a medium-length spacer arm (PEG)2, is membrane-impermeable and exhibits high affinity for free thiol groups on cysteines.
Application
Maleimide-PEG2-biotin is suitable for use in labeling and purifying biomolecules with high specificity. It may be used in mass spectrometry to detect free sulfhydryl residues in intact proteins.
Biochem/physiol Actions
Biotin-PEG2-Mal, a biotin maleimide linker, forms stable thioether bonds by covalently labeling cysteine sulfhydryl groups on antibodies or proteins and can be detected via streptavidin-HRP in Western blot assays following pull-down. Maleimide labels react with sulfhydryl (–SH) groups at acidic pH, minimizing the risk of disulfide bond scrambling during labeling that can happen at higher pH levels.
| SKU | SIAL-SML4178-50MG |
|---|---|
| Supplier Part Number | SML4178-50MG |
| UM | EA |
| UNSPSC | 12352200 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| CAS Number | 305372-39-8 |
| Energy Star | No |
| Green | No |
| Controlled | N |