Cayman Thioredoxin Reductase Colorimetric Assay Kit, 96 wells

Product Description

Cayman’s Thioredoxin Reductase Colorimetric Assay Kit provides a convenient method for detecting TrxR activity in tissue homogenates and cell lysates. It is based on the reduction of DTNB (5,5’-dithio-bis(2-dinitrobenzoic acid); Ellman’s reagent) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) which produces a yellow product that is measured at 405 nm.^1,2 The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity by DTNB reduction in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction (i.e., presence of glutathione).³ The difference between the two results is the DTNB reduction due to TrxR activity.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

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