Cayman Soluble Epoxide Hydrolase Inhibitor Screening Assay Kit, 96 wells

Product Description

Cayman’s fluorescence-based sEH Inhibitor Screening Assay Kit provides a convenient method for screening human sEH inhibitors. The assay utilizes (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) as a substrate. When the epoxide moiety of PHOME is hydrolyzed by sEH, an intramolecular cyclization occurs, which results in the release of a cyanohydrin under basic conditions. The cyanohydrin quickly decomposes into cyanide ion and the highly fluorescent 6-methoxy-2-naphthaldehyde,¹ which can be analyzed using a fluorescence plate reader at excitation and emission wavelengths of 330 and 465 nm, respectively.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

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