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Prostaglandin D2 (PGD2) is biosynthesized in the brain by a lipocalin-type PGD2 synthase where it acts in the CNS to promote sleep induction and lowering of body temperature.1 PGD2 is also the major eicosanoid product of mast cells and is synthesized in large quantities by a hematopoetic-type PGD synthase during allergic and asthmatic anaphylaxis.2,3 Measurement of the parent eicosanoid PGD2 is appropriate in the supernatants of cell cultures, where PGD2 levels may REACH several ng/mL, and in CSF, where concentrations of several hundred pg/mL have been measured.4 Cayman Chemical’s PGD2-MOX Express ELISA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in Cayman’s widely utilized PGD2-MOX ELISA (Item No. 512011). While Item No. 512011 offers superior sensitivity (80% B/B0 ~3.1 pg/mL), the new PGD2-MOX Express EIA offers the convenience of a fast assay while still achieving an 80% B/B0 ~16 pg/mL and an assay range of 7.8-1,000 pg/mL.
Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.
WARNINGThis product is not for human or veterinary use.
