Cayman 13,14-dihydro-15-keto Prostaglandin F2α ELISA Kit, 480 solid wells

Product Description

Prostaglandin F_2α (PGF_2α) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH₂. PGF_2α was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF_2α is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF_2α is rapidly metabolized to 13,14-dihydro-15-keto PGF_2α in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and Δ¹³-reductucase.^1,2,3,4 Measurement of 13,14-dihydro-15-keto PGF_2α in plasma can be used as a marker of the in vivo production of PGF_2α.^5,6 The Cayman Chemical 13,14-dihydro-15-keto PGF_2α Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF_2α within the range of 2.3-5,000 pg/mL, typically with a detection limit (80% B/B₀) of approximately 15 pg/mL. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF_2α in plasma, a common matrix for measurement of this PGF_2α metabolite.⁵

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

Technical Information

Shipping & Storage Information