Cayman LDH Cytotoxicity Assay Kit, 480 wells

Product Description

Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from cell- or compound-induced cytotoxicity. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Caymans LDH Cytotoxicity Assay Kit measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD⁺ to NADH and H⁺ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H⁺ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

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