Cayman α-Ketoglutarate Detection Assay Kit, 96 wells

Product Description

Cayman’s α-kg Detection Assay provides fluorometric- or colorimetric-based methods for quantifying α-kg in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular and extracellular α-kg concentrations in cell culture samples. In this assay, alanine transaminase (ALT) deaminates α-kg to form pyruvate.^1,2 Pyruvate is then oxidized by pyruvate oxidase to yield acetyl phosphate, hydrogen peroxide (H₂O₂), and carbon dioxide.³ As H₂O₂ is produced, the cycling mechanism of horseradish peroxidase (HRP) results in the concurrent reduction of H₂O₂ to H₂O and the oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin.⁴ Resorufin fluorescence is analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

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