Cayman NADPH/NADP Ratio Assay Kit, 96 wells

Product Description

Cayman’s NADPH/NADP Ratio Assay Kit provides a colorimetric method for measuring NADP⁺, NADPH, and their ratio in cell culture extracts. In this assay, NADP⁺ is enzymatically converted to NADPH by glucose-6-phosphate dehydrogenase (G6PDH), which leads to the reduction of the tetrazolium salt substrate WST-8 to a highly colored formazan that absorbs at 450 nm. The amount of formazan produced is proportional to the amount of NADP⁺ and NADPH in the matrix. To measure NADPH specifically, samples are heated to degrade NADP⁺. The NADP⁺ concentration is then calculated by subtracting the signal obtained from the heated sample from that of the corresponding non-heated sample. This assay has a range of 0-1.5 µM and a lower limit of detection (LLOD) of 0.02 µM.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

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