Cell Signaling ATP-Citrate Lyase (D1x6p) Rabbit mAb

Name: Cell Signaling ATP-Citrate Lyase (D1x6p) Rabbit mAb
SKU: CSSPQ-13390S
Price: 351.00 USD

List Price $351.00 Your Price $351.00

Cell Signaling ATP-Citrate Lyase (D1x6p) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-13390S

MFG.PART: 13390S

UNSPSC: 12352300

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	125
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	ATP-Citrate Lyase (D1X6P) Rabbit mAb recognizes endogenous levels of total ATP-citrate lyase protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val34 of human ATP citrate lyase protein.
Background	ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).