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Cell Signaling Rad23b (D4w7f) Rabbit mAb
List Price $351.00 Your Price $351.00
Cell Signaling Rad23b (D4w7f) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-13525S
MFG.PART: 13525S
UNSPSC: 12352202
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 53 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Rad23B (D4W7F) Rabbit mAb recognizes endogenous levels of total Rad23B protein. This antibody does not cross-react with Rad23A protein. |
| Species Reactivity | Human, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala140 of human Rad23B protein. |
| Background | The yeast nucleotide excision repair (NER) radiation sensitive protein 23 (rad23) and its human homologs Rad23A (hHR23A) and Rad23B (hHR23B) are critical components of the cellular machinery that recognize DNA lesions and serve as receptors that target ubiquitinated substrates to the proteasome for degradation (1).The UV excision repair protein Rad23B is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23B contains an amino-terminal ubiquitin-like (UbL) domain that facilitates interaction with the S5a/PSMD4 subunit of the proteasome 19S regulatory complex (2,3). In addition, Rad23B contains a central ubiquitin-associated domain (UBA1) and a carboxy-terminal UBA2 domain, which bind mono- and polyubiquitin with distinct specificities (4). Research studies demonstrate that Rad23B binds specifically to K48-ubiquitinated proteins to facilitate recruitment of target proteins to the proteasome (5). Between the paired UBA domains, Rad23B contains an XPC-binding domain that facilitates binding to XPC and recruitment to DNA lesions (6), as well as the binding of peptide:N-glycanase that is critical for recruitment of ubiquitinated ERAD substrates to the proteasome (7). Research studies have shown that targeted deletion of the murine Rad23b locus impairs embryonic development, suggesting that Rad23B is essential for mammalian development (8). |
| SKU | CSSPQ-13525S |
|---|---|
| Supplier Part Number | 13525S |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |