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Cell Signaling Phospho-Ttf-1 (Ser327) Antibody
List Price
$362.00
Your Price
$362.00
Cell Signaling Phospho-Ttf-1 (Ser327) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-13608S
MFG.PART:
13608S
UNSPSC:
41116111
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 42 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-TTF-1 (Ser327) Antibody recognizes endogenous levels of TTF-1 protein only when phosphorylated at Ser327. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser327 of human TTF-1 protein. |
| Background | Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).Research studies have shown that activation of the Raf/MEK/ERK signaling pathway results in phosphorylation of TTF-1 at multiple residues, including Ser18, Ser327, and Ser337 (7). Concomitant phosphorylation at these residues was shown to suppress the transcriptional activity of TTF-1 in a rat follicular thyroid cell model (7). These findings are consistent with previous studies demonstrating a reduction in thyroid-specific gene expression in Ras-transformed thyroid cells (8,9). |
| SKU | CSSPQ-13608S |
|---|---|
| Supplier Part Number | 13608S |
| UM | EA |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |