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Cell Signaling Phospho-Yap (Ser397) (D1e7y) Rabbit mAb
List Price
$415.00
Your Price
$415.00
Cell Signaling Phospho-Yap (Ser397) (D1e7y) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-13619S
MFG.PART:
13619S
UNSPSC:
41116111
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 65-78 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-YAP (Ser397) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser397. This residue corresponds to Ser381 of YAP isoform 2, as reported by Zhao, B. et Al. (2010) Genes Dev 24, 72-85 (9). |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser397 of human YAP protein isoform 1. |
| Background | YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size (6-8). Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteasomal degradation of YAP (10).Phosphorylation of YAP at Ser397 (annotated as Ser381 by Zhao et Al. 2010) primes YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron (9). Upon phosphorylation, the phosphodegron recruits the β-TrCP (SCF) ubiquitin ligase complex, which ubiquitinates YAP to trigger its proteolytic degradation in the proteasome. |
| SKU | CSSPQ-13619S |
|---|---|
| Supplier Part Number | 13619S |
| UM | EA |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |