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Cell Signaling Phospho-Dapp1/Bam32 (Tyr139) (D7g4g) Rabbit mAb
List Price $415.00 Your Price $415.00
Cell Signaling Phospho-Dapp1/Bam32 (Tyr139) (D7g4g) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-13703S
MFG.PART: 13703S
UNSPSC: 41116124
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 29 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-DAPP1/BAM32 (Tyr139) (D7G4G) Rabbit mAb recognizes endogenous levels of DAPP1/BAM32 protein only when phosphorylated at Tyr139. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr139 of human DAPP1/BAM32 protein. |
| Background | The dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1/BAM32) is a cytoplasmic adaptor protein that mediates the recruitment and interaction of molecules required for signal transduction downstream of the B cell receptor (BCR) (1). The DAPP1/BAM32 protein contains an amino-terminal SH2 domain and a carboxy-terminal pleckstrin homology (pH) domain that binds to PI3K-derived phosphoinositides (i.e., PIP3). Upon BCR activation, DAPP1/BAM32 is phosphorylated at specific tyrosine residues and translocated from the cytoplasm to the membrane. Research studies indicate that phosphorylation and translocation of DAPP1/BAM32 is strongly dependent upon PI3K signaling (2,3). The amino-terminal SH2 domain binds to PLCγ2 and other tyrosine-phosphorylated targets. As a result of these interactions, DAPP1/BAM32 can adjust the response to receptor activation by coordinating membrane-localized interactions among proteins of distinct signal transduction pathways (1,4). DAPP1/BAM32 is expressed most abundantly in B lymphocytes; high expression during dendritic cell (DC) maturation and localization to contact sites between DC and allogenic T cells suggest that the DAPP1/BAM32 adaptor may play a role in the activation of T cells through MHC class I-mediated signaling pathways (5).Research studies show that phosphorylation of DAPP1/BAM32 at Tyr139 is PI3K-dependent, requires an intact pH domain in DAPP1/BAM32, and is likely performed by Src-family kinases following membrane recruitment of DAPP1/BAM32 by phosphoinositides (6). Blocking phosphorylation of DAPP1/BAM32 at Tyr139 inhibits BCR internalization and reduces cellular F-actin levels, suggesting that phosphorylation of DAPP1/BAM32 may play a role in regulating actin-dependent internalization of the activated BCR (7,8). |
| SKU | CSSPQ-13703S |
|---|---|
| Supplier Part Number | 13703S |
| UM | EA |
| UNSPSC | 41116124 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |