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Cell Signaling Acetyl-Histone H3 (Lys18) (D8z5h) Rabbit mAb
List Price $427.00 Your Price $427.00
Cell Signaling Acetyl-Histone H3 (Lys18) (D8z5h) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-13998S
MFG.PART: 13998S
UNSPSC: 41106509
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk Sc |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 17 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200, Immunohistochemistry (Paraffin): 1:50 - 1:200, Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200, Chromatin IP: 1:50, Chromatin IP-seq: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Acetyl-Histone H3 (Lys18) (D8Z5H) Rabbit mAb recognizes endogenous levels of histone H3 protein only when acetylated at Lys18. This antibody does not cross-react with other acetylated histone proteins. |
| Species Reactivity | Human, Mouse, Rat, Monkey, S. cerevisiae |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding acetylated Lys18 of human histone H3 protein. |
| Background | The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, 36, and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the accessibility of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues (6). Many transcription and chromatin regulatory proteins contain bromodomains and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF, and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation (3). Deacetylation, which is mediated by histone deacetylases (HDAC and sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression (7,8). |
| SKU | CSSPQ-13998S |
|---|---|
| Supplier Part Number | 13998S |
| UM | EA |
| UNSPSC | 41106509 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |