Cell Signaling Cleaved Caspase-8 (Asp387) (D5b2) Xp Rabbit mAb (Mouse Specific) (PE Conjugate)

Name: Cell Signaling Cleaved Caspase-8 (Asp387) (D5b2) Xp Rabbit mAb (Mouse Specific) (PE Conjugate)
SKU: CSSPQ-14071S
Price: 493.00 USD

List Price $493.00 Your Price $493.00

Cell Signaling Cleaved Caspase-8 (Asp387) (D5b2) Xp Rabbit mAb (Mouse Specific) (PE Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-14071S

MFG.PART: 14071S

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, the unconjugated antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43) by western blot analysis.
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.
Background	Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.