Cell Signaling Caspase-3 (D3r6y) Rabbit mAb

Name: Cell Signaling Caspase-3 (D3r6y) Rabbit mAb
SKU: CSSPQ-14220T
Price: 152.00 USD

List Price $152.00 Your Price $152.00

Cell Signaling Caspase-3 (D3r6y) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-14220T

MFG.PART: 14220T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	35, 19, 17
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Caspase-3 (D3R6Y) Rabbit mAb recognizes endogenous levels of total caspase-3 protein. This antibody detects full-length caspase-3 as well as the large subunit (p20) of caspase-3 resulting from cleavage during apoptosis.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-3 protein.
Background	Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).