Cell Signaling Phospho-Cdc2 (Tyr15) (10a11) Rabbit mAb (PE Conjugate)

Name: Cell Signaling Phospho-Cdc2 (Tyr15) (10a11) Rabbit mAb (PE Conjugate)
SKU: CSSPQ-14566S
Price: 493.00 USD

List Price $493.00 Your Price $493.00

Cell Signaling Phospho-Cdc2 (Tyr15) (10a11) Rabbit mAb (PE Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-14566S

MFG.PART: 14566S

UNSPSC: 41116111

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M R Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (PE Conjugate) detects endogenous levels of cdc2 protein only when phosphorylated at Tyr15. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2.
Background	The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).