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Cell Signaling Phospho-Csf-1r/M-Csf-R (Tyr708) (D5f4y) Rabbit mAb
List Price
$415.00
Your Price
$415.00
Cell Signaling Phospho-Csf-1r/M-Csf-R (Tyr708) (D5f4y) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-14591S
MFG.PART:
14591S
UNSPSC:
41116133
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 175 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-CSF-1R/M-CSF-R (Tyr708) (D5F4Y) Rabbit mAb recognizes endogenous levels of CSF-1R/M-CSF-R only when phosphorylated at Tyr708. This antibody may cross-react with other activated protein tyrosine kinases such as phospho-Src. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr708 of human CSF-1R/M-CSF-R protein. |
| Background | Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).Tyr708 (Tyr706 in mouse) is located in the KI region of M-CSF receptor. Phosphorylation of Tyr708 may influence the binding of PI3 kinase to the activated M-CSF receptor (9). |
| SKU | CSSPQ-14591S |
|---|---|
| Supplier Part Number | 14591S |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |