Cell Signaling Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65e4) Rabbit mAb (PE Conjugate)

Name: Cell Signaling Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65e4) Rabbit mAb (PE Conjugate)
SKU: CSSPQ-14791S
Price: 493.00 USD

List Price $493.00 Your Price $493.00

Cell Signaling Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65e4) Rabbit mAb (PE Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-14791S

MFG.PART: 14791S

UNSPSC: 41116124

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (PE Conjugate) detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70 protein.
Background	The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

Specifications