Cell Signaling Spt6 (D6j9h) Rabbit mAb

Name: Cell Signaling Spt6 (D6j9h) Rabbit mAb
SKU: CSSPQ-15616S
Price: 351.00 USD

List Price $351.00 Your Price $351.00

Cell Signaling Spt6 (D6j9h) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-15616S

MFG.PART: 15616S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	210
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200, Chromatin IP: 1:50, CUT&RUN: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	SPT6 (D6J9H) Rabbit mAb recognizes endogenous levels of total SPT6 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val112 of human SPT6 protein.
Background	SPT6 or SUPT6H is a histone H3 chaperone protein involved in transcriptional elongation and chromatin structure (1). The SPT6 protein contains a highly acidic N-terminus with leucine zipper and SH2 domains, which can interact with phospho-Rpb1 CTD (Ser2) to recruit SPN1 and other mRNA processing and export factors (2). SPT6 can enhance the elongation rate of RNA polymerase II, and can also maintain the modification state of histone H3 tails. (3-4). Loss of SPT6 causes improper initiation of transcription within coding regions (5).

Specifications