Cell Signaling Atg12 Antibody (Human Specific)

Name: Cell Signaling Atg12 Antibody (Human Specific)
SKU: CSSPQ-2010T
Price: 136.00 USD

List Price $136.00 Your Price $136.00

Cell Signaling Atg12 Antibody (Human Specific) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2010T

MFG.PART: 2010T

UNSPSC: 41116133

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	16, 55
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Atg12 Antibody detects endogenous levels of total free and Atg5 bound Atg12 protein.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Atg12. Antibodies are purified by peptide affinity chromatography.
Background	Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).