Cell Signaling Axin1 (C95h11) Rabbit mAb

Name: Cell Signaling Axin1 (C95h11) Rabbit mAb
SKU: CSSPQ-2074S
Price: 348.00 USD

List Price $348.00 Your Price $348.00

Cell Signaling Axin1 (C95h11) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2074S

MFG.PART: 2074S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	110
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Axin1 (C95H11) Rabbit mAb detects the endogenous levels of total Axin1 protein.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant protein of human Axin1.
Background	Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).