Cell Signaling Non-Phospho-Src (Tyr416) (7g9) Mouse mAb

Name: Cell Signaling Non-Phospho-Src (Tyr416) (7g9) Mouse mAb
SKU: CSSPQ-2102T
Price: 169.00 USD

List Price $169.00 Your Price $169.00

Cell Signaling Non-Phospho-Src (Tyr416) (7g9) Mouse mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2102T

MFG.PART: 2102T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Mouse IgG2b
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Non-phospho-Src (Tyr416) (7G9) Mouse mAb detects endogenous levels of Src only when dephosphorylated at tyrosine 416. The antibody cross-reacts with other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr419 of human Src.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).