Cell Signaling Phospho-Src (Tyr527) Antibody

Name: Cell Signaling Phospho-Src (Tyr527) Antibody
SKU: CSSPQ-2105T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Phospho-Src (Tyr527) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2105T

MFG.PART: 2105T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Src (Tyr527) Antibody detects endogenous levels of Src only when phosphorylated at Tyr527. The antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr530 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).