Cell Signaling Non-Phospho-Src (Tyr527) Antibody

Name: Cell Signaling Non-Phospho-Src (Tyr527) Antibody
SKU: CSSPQ-2107T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Non-Phospho-Src (Tyr527) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2107T

MFG.PART: 2107T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Non-phospho-Src (Tyr527) Antibody detects endogenous levels of Src only when dephosphorylated at tyrosine 527. It may also cross-react with Yes, Fyn and Slk based on sequence similarity.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr530 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).