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Cell Signaling Phospho-Estrogen Receptor Alpha (Ser118) (16j4) Mouse mAb
List Price $167.00 Your Price $167.00
Cell Signaling Phospho-Estrogen Receptor Alpha (Ser118) (16j4) Mouse mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-2511T
MFG.PART: 2511T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 66 |
| Source/Isotype | Mouse IgG2b |
| Application/Dilution | {Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:800} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb detects endogenous levels of estrogen receptor α only when phosphorylated at serine 118. It does not cross-react with phosphorylated estrogen receptor β. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser118 of human ER α. |
| Background | Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4). |
| SKU | CSSPQ-2511T |
|---|---|
| Supplier Part Number | 2511T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |