Cell Signaling Phospho-Cdc2 (Thr14) Antibody

Name: Cell Signaling Phospho-Cdc2 (Thr14) Antibody
SKU: CSSPQ-2543S
Price: 362.00 USD

List Price $362.00 Your Price $362.00

Cell Signaling Phospho-Cdc2 (Thr14) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2543S

MFG.PART: 2543S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H Hm Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	34
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-cdc2 (Thr14) Antibody detects endogenous levels of cdc2 (CDK1) only when phosphorylated at Thr14. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3.
Species Reactivity	Human, Hamster, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr14 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

Specifications