Cell Signaling Pcna (Pc10) Mouse mAb

Name: Cell Signaling Pcna (Pc10) Mouse mAb
SKU: CSSPQ-2586T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Pcna (Pc10) Mouse mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2586T

MFG.PART: 2586T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Mk B Pg
Sensitivity	Endogenous
Molecular Weight (kDa)	36
Source/Isotype	Mouse IgG2a
Application/Dilution	{Western Blotting: 1:2000, Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:8000 - 1:32000, Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200, Flow Cytometry (Fixed/Permeabilized): 1:400}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.
Species Reactivity	Human, Mouse, Rat, Monkey, Bovine, Pig
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.
Background	Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.

Specifications