Cell Signaling Cul4a Antibody

Name: Cell Signaling Cul4a Antibody
SKU: CSSPQ-2699T
Price: 136.00 USD

List Price $136.00 Your Price $136.00

Cell Signaling Cul4a Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2699T

MFG.PART: 2699T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	80, 82
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	CUL4A Antibody detects endogenous levels of total human CUL4A protein.
Species Reactivity	Human, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Ser12 of human CUL4A. Antibodies are purified by peptide affinity chromatography.
Background	Cullin-4 (CUL4) is a member of the cullin family of related ubiquitin ligases (1). A pair of mammalian CUL4 proteins - CUL4A and CUL4B - has been identified. The carboxy-terminal domain of CUL4 interacts with Rbx1 and E2 enzyme while the amino-terminal CUL4 domain interacts with BPB domain of UV-damaged DNA binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex (2). This CUL4-DDB1 complex binds a variety of WD40-containing proteins that help to enhance additional substrate recruitment (2,3). The CUL4 complex has been shown to target substrates involved in DNA repair, cell cycle progression (2), transcription (3), and development (2-6).