Cell Signaling Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody

Name: Cell Signaling Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody
SKU: CSSPQ-2704T
Price: 150.00 USD

List Price $150.00 Your Price $150.00

Cell Signaling Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2704T

MFG.PART: 2704T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	70
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Zap-70 (Tyr493)/Syk (Tyr526) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr493. This antibody may cross-react with other activated tyrosine kinases.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr493 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).Phosphorylation of Tyr493 within the activation loop results in enzymatic activation of Zap-70 (9).