Cell Signaling Lck (V49) Antibody

Name: Cell Signaling Lck (V49) Antibody
SKU: CSSPQ-2714S
Price: 317.00 USD

List Price $317.00 Your Price $317.00

Cell Signaling Lck (V49) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-2714S

MFG.PART: 2714S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	56
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Lck (V49) Antibody detects endogenous levels of total Lck protein. This antibody does not cross-react with other src family proteins.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val49 of human Lck. Antibodies are purified by peptide affinity chromatography.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).Lck is essential for T-lymphocyte activation and differentiation (3,4). Phosphorylation of Tyr505 in the carboxy-terminal tail of Lck downregulates its catalytic activity, while phosphorylation of Tyr394 leads to an increase in Lck activity (5).