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Cell Signaling Phospho-Lyn (Tyr507) Antibody
List Price $148.00 Your Price $148.00
Cell Signaling Phospho-Lyn (Tyr507) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-2731T
MFG.PART: 2731T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 53, 56 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-Lyn (Tyr507) Antibody detects endogenous levels of Lyn only when phosphorylated at Tyr507. The antibody may cross-react with phospho-Lck (Tyr505) and phospho-Src (Tyr527) due to high sequence homology. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr507 of human Lyn. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8). |
| SKU | CSSPQ-2731T |
|---|---|
| Supplier Part Number | 2731T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |