Cell Signaling Cdc2 (E1z6r) Rabbit mAb

Name: Cell Signaling Cdc2 (E1z6r) Rabbit mAb
SKU: CSSPQ-28439S
Price: 351.00 USD

List Price $351.00 Your Price $351.00

Cell Signaling Cdc2 (E1z6r) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-28439S

MFG.PART: 28439S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Hm Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	34
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cdc2 (E1Z6R) Rabbit mAb recognizes endogenous levels of total cdc2 protein.
Species Reactivity	Human, Mouse, Rat, Hamster, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp289 of human cdc2 protein.
Background	The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).