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Cell Signaling Xlf Antibody
List Price $136.00 Your Price $136.00
Cell Signaling Xlf Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-2854T
MFG.PART: 2854T
UNSPSC: 41116111
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 39 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | XLF Antibody detects endogenous levels of total XLF protein. |
| Species Reactivity | Human |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human XLF. Antibodies are purified by peptide affinity chromatography. |
| Background | Double stranded DNA breaks (DSB’s) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. The mechanism by which cells deal with DSB’s is known as NHEJ (non-homologous end-joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1). XLF, also known as Cernunnos, was originally discovered as a mutated protein from cells of individuals who displayed features of growth retardation, microcephaly, and immunodeficiency (2). These cells were sensitive to ionizing radiation and defective in V(D)J recombination. Exogenous expression of wild type XLF corrected these deficiencies (3), indicating that XLF is a critical component of the NHEJ response. XLF physically interacts with and may stimulate the ligase activity of XRCC4 (3). |
| SKU | CSSPQ-2854T |
|---|---|
| Supplier Part Number | 2854T |
| UM | EA |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |