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Cell Signaling Phospho-Nf-Kappab P65 (Ser536) (93h1) Rabbit mAb
List Price $170.00 Your Price $170.00
Cell Signaling Phospho-Nf-Kappab P65 (Ser536) (93h1) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-3033T
MFG.PART: 3033T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H M R Hm Mk Pg |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 65 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200, Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #76778. |
| Specificity/Sensitivity | Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb detects NF-κB p65 only when phosphorylated at Ser536. It does not cross-react with the p50 subunit or other related proteins. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Pig |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65. |
| Background | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
| SKU | CSSPQ-3033T |
|---|---|
| Supplier Part Number | 3033T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |